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Literature summary for 2.4.2.2 extracted from
- Substrate specificity of E. coli uridine phosphorylase. Further evidences of high-syn conformation of the substrate in uridine phosphorolysis (2017), Nucleosides Nucleotides Nucleic Acids, 36, 107-121 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | linear regression analysis of the Michaelis-Menten and Lineweaver-Burk plots | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uridine + phosphate | Escherichia coli | - |
uracil + alpha-D-ribose 1-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0C037 | pyrimidine/purine nucleoside phosphorylase | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-C-methyluridine + phosphate | - |
Escherichia coli | uracil + 2-C-methyl-alpha-D-ribose 1-phosphate | - |
r | |
| 2'-deoxyuridine + phosphate | - |
Escherichia coli | uracil + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 3'-C-methyluridine + phosphate | - |
Escherichia coli | uracil + 3-C-methyl-alpha-D-ribose 1-phosphate | - |
r | |
| 3'-fluoro-3'-deoxyuridine + phosphate | - |
Escherichia coli | uracil + 3'-fluoro-3'-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 4-thiouridine + phosphate | - |
Escherichia coli | 4-thiouracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5'-azido-5'-deoxythymidine + phosphate | - |
Escherichia coli | thymine + 5-azido-5-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-bromo-5'-deoxythymidine + phosphate | - |
Escherichia coli | thymine + 5-bromo-5-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-chloro-5'-deoxythymidine + phosphate | - |
Escherichia coli | thymine + 5'-chloro-5'-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-deoxy-5'-methyleneuridine + phosphate | - |
Escherichia coli | uracil + 5-deoxy-5'-methylene-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-deoxythymidine + phosphate | - |
Escherichia coli | thymine + 5-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-deoxyuridine + phosphate | - |
Escherichia coli | uracil + 5-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-homothymidine + phosphate | - |
Escherichia coli | thymine + 5-homo-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-homouridine + phosphate | - |
Escherichia coli | uracil + 5-homo-alpha-D-ribose 1-phosphate | - |
r | |
| 5'-iodo-5'-deoxythymidine + phosphate | - |
Escherichia coli | thymine + 5-iodo-5-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5-C-methyluridine + phosphate | - |
Escherichia coli | 5-C-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | - |
Escherichia coli | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 6-methyluridine + phosphate | - |
Escherichia coli | 6-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| additional information | kinetic constants of the phosphorolysis reaction of uridine derivatives modified at 2'-, 3'- and 5'-positions of the sugar moiety and 2-, 4-, 5- and 6-positions of the heterocyclic base are determined. The absence of the 2'- or 5'-hydroxyl group is not crucial for successful binding and phosphorolysis. On the other hand, absence of both the 2'- and 5'-hydroxyl groups leads to loss of substrate binding to the enzyme. Substrate specificity, overview. 2,2'-O-Anhydrouridine, that models the high-syn conformation of uridine, binds better to the enzyme than uridine, despite the absence of a proton in the 3-position of the heterocyclic base, thus playing an important role in the substrate binding. In this case, the orientation of the heterocycle substantially outweighs the contribution of hydrogen bonding by the 2'-hydroxyl and 3-NH groups in the active site of enzyme UP. This strongly suggests that the 2-oxo group is located quite close to 2'-H in the substrate-UP complex. This is in line with the lack of substrate properties of 2-thiouridine | Escherichia coli | ? | - |
- |
|
| uridine + phosphate | - |
Escherichia coli | uracil + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 27500 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyrimidine/purine nucleoside phosphorylase | UniProt | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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